Brilliant green is a synthetic dye and antiseptic agent used in microbiology. It is effective against Gram-positive bacteria and has been used in medical and veterinary applications.
| IUPAC Name | [4-[[4-(diethylamino)phenyl]-phenylmethylidene]cyclohexa-2,5-dien-1-ylidene]-diethylazanium;hydrogen sulfate |
| Molecular Formula | C27H34N2O4S |
| CAS Number | 633-03-4 |
| Synonyms | Malachite green G, Basic green 1, Diamond green B, New green, Solid green, Aniline green, Fast green, Victoria green B, Solidum, Lumin green |
| InChI | InChI=1S/C27H34N2O4S/c1-19-8-10-23(18-26(19)34(31,32)33-3)28-16-14-25(15-17-28)29(2)24-11-9-20(21(12-24)13-27(30)35)22-6-4-5-7-22/h4-13,18H,14-17H2,1-3H3/q+1 |
Brilliant Green molar mass
Brilliant Green has a molar mass of approximately 482.64 g/mol. This value is calculated by summing the atomic masses of all the atoms present in one molecule of Brilliant Green. It is an important parameter for determining the amount of substance in a given sample of Brilliant Green. Molar mass is often used in chemical reactions and stoichiometry calculations to determine the number of reactants or products needed.
Brilliant Green boiling point
The boiling point of Basic Green is approximately 410-415 °C (770-779 °F). Boiling point is the temperature at which the vapor pressure of a liquid equals the atmospheric pressure, causing the liquid to boil and turn into a gas. The high boiling point of Basic Green indicates that it has a strong intermolecular force between its molecules, requiring high energy to separate them into individual molecules.
Brilliant Green melting point
The melting point of Basic Green is approximately 218-220 °C (424-428 °F). Melting point is the temperature at which a solid substance changes into a liquid state. The melting point of Basic Green is relatively high, indicating strong intermolecular forces that hold the molecules together in a solid state.
Basic Green density g/ml
The density of Basic Green is approximately 1.2-1.3 g/ml at room temperature. Density is the mass of a substance per unit volume and is often used to characterize materials in various industries. The density of Basic Green suggests that it is a relatively dense substance compared to water, which has a density of 1 g/ml.
Basic Green molecular weight
The molecular weight of Basic Green is approximately 482.64 g/mol. Molecular weight is the sum of the atomic weights of all atoms in a molecule. It is an important parameter in determining the physical and chemical properties of a substance, as well as in stoichiometry calculations.
Basic Green Structure
Basic Green has a complex structure consisting of several functional groups. It has a 1,3,5-oxadiazinane ring system, which contains two nitrogen atoms and one oxygen atom. The ring system is attached to a phenyl ring and two methyl groups, and there is also a quaternary ammonium cation present in the molecule. The complex structure of Basic Green contributes to its unique chemical and physical properties, making it useful in various applications.
Basic Green formula
The chemical formula of Basic Green is C27H34N2O4S. This formula indicates the number and type of atoms present in the molecule of Basic Green. The formula is used to calculate the molar mass and other important properties of the substance.
| Appearance | Green crystalline powder |
| Specific Gravity | 1.2-1.3 g/mL |
| Color | Green |
| Odor | Odorless |
| Molar Mass | 482.64 g/mol |
| Density | 1.2-1.3 g/mL |
| Melting Point | 218-220 °C |
| Boiling Point | 410-415 °C |
| Flash Point | Not applicable |
| Water Solubility | Slightly soluble |
| Solubility | Soluble in alcohol, chloroform, and ether |
| Vapour Pressure | Not available |
| Vapour Density | Not available |
| pKa | Not available |
| pH | Not available |
Brilliant Green Safety and Hazards
Brilliant Green may pose hazards to human health if not handled properly. It may cause skin and eye irritation upon contact and may be harmful if ingested. Inhaling the substance may also cause respiratory irritation. Prolonged or repeated exposure to Brilliant Green may lead to adverse health effects. It is important to follow proper safety precautions when handling Brilliant Green, such as wearing appropriate personal protective equipment and avoiding direct contact with the substance. In case of exposure or ingestion, seek medical attention immediately. It is also important to store and dispose of Brilliant Green properly to prevent harm to people and the environment.
| Hazard Symbols | Xi |
| Safety Description | Avoid contact with skin and eyes. In case of contact, rinse thoroughly with water and seek medical attention. Wear appropriate personal protective equipment. |
| UN Ids | Not applicable |
| HS Code | 3204.19.00 |
| Hazard Class | 6.1 |
| Packing Group | III |
| Toxicity | Harmful if ingested. May cause skin and eye irritation. Prolonged or repeated exposure may cause adverse health effects. |
Brilliant Green Synthesis Methods
There are various methods to synthesize Brilliant Green (C27H34N2O4S) including:
- One method involves reacting malachite green (C23H25N2Cl) with sulfuric acid (H2SO4) and sodium nitrite (NaNO2) at low temperatures, adding sodium acetate (NaOAc) and acetic anhydride (C4H6O3), and heating and refluxing the mixture to form Brilliant Green.
- Another method involves reacting N,N-dimethylaniline (C8H11N) with ethyl oxalyl chloride (C6H5ClO2) in the presence of aluminum chloride (AlCl3) and carbon disulfide (CS2), followed by adding sodium hydroxide (NaOH) and formaldehyde (CH2O), treating with sulfuric acid and sodium nitrite, and adding sodium acetate and acetic anhydride to produce Brilliant Green.
- To form Brilliant Green, one can react malachite green with sodium nitrite and sulfuric acid in the presence of sodium acetate and acetic anhydride. After that, heat and reflux the resulting mixture.
Brilliant Green Uses
Brilliant Green (C27H34N2O4S) has a range of uses across various fields. Some of the key uses of basic green include:
- Staining bacteria, mitochondria, and other organelles for biological microscopy.
- Acting as an indicator for determining reducing sugars and identifying certain alkaloids.
- Treating external infections like pyodermas and otitis externa and serving as an antiseptic in veterinary medicine.
- Dyeing wool and silk as a textile dye.
- Regulating plant growth and controlling plant diseases as a fungicide in agriculture.
- Acting as a selective agent in culture media for biological research.
- Treating wounds and infections in veterinary medicine as an antiseptic agent.
- Serving as a food colorant in the food industry.
These applications highlight the versatility of Brilliant Green and its importance in various fields. However, it is important to follow proper safety protocols when using Brilliant Green to prevent harm to people and the environment.